Hepatitis C Virus HCV is a diverse human pathogen which displays 15 divergence at the subtype level To facilitate development of antivirals with pan genotype activity we developed the

Establishment of robust HCV genotype 4d 5a and 6a replicon systems Sign in Create an account https orcid org Europe PMC Menu About About Europe PMC

In this study we recovered genotype 5a full length genomes from four infected voluntary blood donors in South Africa and established a G418 selectable subgenomic replicon system using one of these strains The replicon derived from the wild type sequence failed to replicate in Huh 7 5 cells

Establishment of robust HCV genotype 4d 5a and 6a replicon

Establishment of infectious genotype 4 cell culture derived hepatitis C virus An infectious ED43 strain containing adaptive mutations is established which is important for the analysis of HCV genotype specific pathogenesis development of pan genotypic agents and analysis of drug resistance

Here we describe the generation of robust genotype 3 sub genomic replicons SGRs based on the adapted HCV NS3 NS5B replicase from the DBN3a cell culture infectious clone Such infectious cell culture adaptive mutations could potentially promote the development of robust SGRs for other HCV strains and genotypes

Establishment of robust HCV genotype 4d 5a and 6a replicon

HCV Replicon Systems Hepatitis C Viruses NCBI Bookshelf

Establishment Of Robust Hcv Genotype 4d 5a And 6a Replicon

Hepatitis C Virus HCV is a diverse human pathogen which displays 15 divergence at the subtype level To facilitate development of antivirals with pan genotype activity we developed the first genotype 4d subgenomic replicon as well as new replicons for genotypes 5a and 6a

Robust and Persistent Replication of the Genotype 6a

Development of an Infectious Cell Culture System for

Finally in 1999 and after years of intensive research the first robust in vitro HCV replicon model based on a genotype 1b clone transfected into a hepatoma cell line was established

Breakthroughs in hepatitis C research from discovery to cure

HCV replicons have been the primary tools for discovering and evaluating the inhibitory activity of DAAs against viral replication Interest in replicon systems has further grown as they have become indispensable for discovering genotype specific and cross genotype RASs

Hepatitis C virus genotype 4 A poorly characterized endemic

Globally 13 of all hepatitis C virus HCV infections are caused by genotype 4 GT4 which consists of 17 subtypes with various levels of susceptibility to anti HCV therapy This genotype is endemic in the Middle East and Africa and has considerably spread to Europe lately

Hepatitis C virus HCV is classified into seven major genotypes and genotype 6 is commonly prevalent in Asia thus reverse genetic system representing genotype 6 isolates in prevalence is required Here we developed an infectious clone for a Chinese HCV 6a isolate CH6a using a novel strategy

Genotyping diagnostic methods for hepatitis C virus A need

These new adaptive mutations allowed establishment of robust luciferase encoding GT6a replicons for reproducible quantification of HCV replication and the luciferase encoding replicons enabled efficient determinations of antiviral activity for HCV inhibitors in a 384 well assay format

Establishment of robust HCV genotype 4d 5a and 6a replicon

Many improvements to the replicon system have been made allowing the establishment of transient replication assays for HCV genotypes 1a 1b and 2a

Robust and Persistent Replication of the Genotype 6a

Hepatitis C Virus HCV is a diverse human pathogen which displays 15 divergence at the subtype level To facilitate development of antivirals with pan genotype activity we developed the first

HCV genotype kit cobas HCV GT manufactured by Roche claims to identify HCV genotypes 1 6 and can discriminate between subtype a and b of genotype 1 Fully automated system cobas 4800 Roche USA has also been launched for HCV genotyping

Hepatitis C Virus HCV is a diverse human pathogen which displays 15 divergence at the subtype level To facilitate development of antivirals with pan genotype activity we developed the first

Insights into the unique characteristics of hepatitis C virus

Julia LU Laboratory Assistant Gilead Sciences Foster

Hepatitis C Virus Genotype 5a Subgenomic Replicons for

Establishment of infectious genotype 4 cell culture derived

Establishment of robust HCV genotype 4d 5a and 6a replicon

Establishment Of Robust Hcv Genotype 4d 5a And 6a Replicon

To establish infectious genotype 4a GT4a cell culture derived hepatitis C virus HCVcc we constructed full length ED43 and 12 mutants possessing single or double mutations that increase ED43 replicon replication and performed cell culture after RNA transfection

Michael D Miller 39 s research works Gilead Sciences Foster

Establishment of robust HCV genotype 4d 5a and 6a replicon

HCV Replicon Systems Workhorses of Drug Discovery and

To facilitate development of antivirals with pan genotype activity we developed the first genotype 4d subgenomic replicon as well as new replicons for genotypes 5a and 6a Adaptive mutations developed in these replicons differ greatly across genotypes